Part:BBa_K1351022

FsrA: Fur-regulated sRNA which binds the binding site sdhC

This part was generated with the RFC10 standard and has the following prefix and suffix:

prefix with EcoRI, NotI and XbaI:	GAATTCGCGGCCGCTTCTAGAG
suffix with SpeI, NotI and PstI:	TACTAGTAGCGGCCGCTGCAG

Sites of restriction enzymes generating compatible overhangs have the same color: EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red.

This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].

Results: [http://2014.igem.org/Team:LMU-Munich/Results LMU-2014-Results]

This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].

FsrA: a small RNA Represses Expression of Succinate Dehydrogenase

Fig. 1. Predicted pairing between fsrA and the 5’-leader region of sdhC, the first gene of the sdhCAB operon.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]