Coding
lacI

Part:BBa_C0012

Designed by: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton   Group: Antiquity   (2003-01-31)
Revision as of 01:49, 30 April 2006 by Registry (Talk | contribs)


lacI repressor from E. coli (+LVA)

Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator BBa_R0010 and PLlac01 hybrid regulator BBa_R0011 and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription.

A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.

Usage and Biology

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Sequence and Features


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1147
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Functional Parameters

directionForward
functionRep
ligandsallolactose, IPTG
proteinLacI
swissproP03023
tagLVA
[edit]
Categories
//cds/transcriptionalregulator/repressor
//function/regulation/transcriptional
Parameters
directionForward
functionRep
ligandsallolactose, IPTG
proteinLacI
swissproP03023
tagLVA