Coding

Part:BBa_K1321364:Design

Designed by: Chris N Micklem   Group: iGEM14_Imperial   (2014-10-08)
Revision as of 20:10, 24 October 2014 by Gabi1234 (Talk | contribs) (→‎Source)

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NiBP fused to dCBD with linker driven by T7


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 53
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 232


Design Notes

The fusion protein was cloned together using the NgoMIV and AgeI sites.

The promotor was cloned onto the protein via the XbaI/SpeI sites.

Source

Our NiBP came from BBa_K1151001.

The T7 vector was made by inverse PCR of BBa_I746909, and ligated. Please see our BBa_ K1321338 for more information on this part.

dCBD with its linker were synthesized from Geneart and cloned into the psB1C3 backbone.

References