Coding

Part:BBa_K1321362:Design

Designed by: Chris N Micklem   Group: iGEM14_Imperial   (2014-10-08)
Revision as of 20:05, 24 October 2014 by Gabi1234 (Talk | contribs) (→‎Design Notes)

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sfGFP fused to CBDcex driven by LacI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 230
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 245


Design Notes

The fusion protein was cloned together using the NgoMIV and AgeI sites.

The promotor was cloned onto the protein via the XbaI/SpeI sites.

Source

Our sfGFP part was an improvement of BBa_I746909, wherein Freiburg sites were added. Please see BBa_K1321337 for more info on this part.

CBDcex was requested from the registry, as it was submitted by the Freiburg team as BBa_K863101.

The LacI part came from BBa_J04500.

References