Terminator

Part:BBa_B0013

Designed by: Reshma Shetty   Group: Antiquity   (2003-01-31)
Revision as of 01:49, 30 April 2006 by Registry (Talk | contribs)


TE from coliphage T7 (+/-)

Strong transcriptional terminator consisting of a 20 bp stem-loop that has been engineered to be bidirectional.

Usage and Biology

<img src="Include File TE.gif" width="413" height="246">

Above is a picture of the wildtype terminator. This terminator has been altered, see design notes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Functional Parameters

biologyT7TE (+/-)
directionForward
forward_efficiency0.6[CC]
reversed_version591
reverse_efficiency-1.06[CC]
[edit]
Categories
//direction/forward
//terminator/single
Parameters
biologyT7TE (+/-)
directionForward
forward_efficiency0.6[CC]
reversed_version591
reverse_efficiency-1.06[CC]