Part:BBa_J37015:Experience

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User Reviews

UNIQ9a8734d964235329-partinfo-00000000-QINU UNIQ9a8734d964235329-partinfo-00000001-QINU

This part was tested by the Brown iGEM team in August 2007. Some interesting and very repeatable results were obtained. It was expected that this part, J37015, would produce significantly more GFP than the part T9002, which differs only in that it has no LuxI gene and therefore no positive feedback loop. It was also expected that higher amounts of AHL would cause J37015 to produce more GFP than low amounts of AHL. Both of these hypotheses were proven false.

The raw data for our experiments can be seen here: 8-14-07_amplifier_test.xls

UNIQ9a8734d964235329-partinfo-00000002-QINU

••••• [http://openwetware.org/wiki/IGEM:IMPERIAL/2006 IGEM:IMPERIAL/2006]

The [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Protocols/J37015 protocol for J37015] was largely based on the [http://openwetware.org/wiki/Endy:F2620/Transfer_Curve/Protocols protocol by Drew Endy and Barry Canton] for the receiver BBa_F2620. Results and analysis for this part can be found [http://openwetware.org/wiki/IGEM:Imperial/Results/J37015 here].

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