Coding

Part:BBa_K1321110:Design

Designed by: Xenia Spencer-Milnes   Group: iGEM14_Imperial   (2014-10-09)
Revision as of 20:17, 21 October 2014 by Gabi1234 (Talk | contribs) (→‎Source)


Phytochelatin (PC) EC20 fused to linker-dCBD with LacI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 230
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker. The LacI vector was fused to the protein fusion using the Xba and SpeI site.

Source

Phytochelatin and dCBD with its linker were synthesized from Geneart and cloned into the psB1C3 backbone. The LacI part was made by inverse PCR of BBa_J04500, and ligated.

References