Coding

Part:BBa_K1321100:Design

Designed by: Xenia Spencer-Milnes   Group: iGEM14_Imperial   (2014-10-09)
Revision as of 19:43, 21 October 2014 by Gabi1234 (Talk | contribs) (→‎Design Notes)

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Phytochelatin (PC) EC20 fused to linker-dCBD with T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 53
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimised for E.coli. Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker.

Source

Phytochelatin and dCBD with its linker were synthesized from Geneart and cloned into the psB1C3 backbone.

References