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Coding

Part:BBa_K1456001:Design

Designed by: Mustafa Semih Elitok   Group: iGEM14_ATOMS-Turkiye   (2014-07-21)
Revision as of 14:20, 21 October 2014 by Mustafa26 (Talk | contribs)

Human Tissue Plasminogen Activator (htPA)

ATOMS-tpa results1.png

Cloning

ATOMS-tpa results3.png

  • Through the primers that we ordered and the cDNA, we acquired tPA inserts. The insert was, as expected, portraying that the base length was around 1700 bp.

File:Https://static.igem.org/mediawiki/2014/0/0c/ATOMS-tpa results5.png

  • The specified primers were put into colony PCR. As it can be seen in the results above, a right insert was not achieved. This process was repeated several times but no result was achieved. Seeing that ligation did not provide a solution to the problem, synthetically produced inserts were ordered.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 5
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 119
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 958

Design Notes

None

Source

We clonned this part from human hepatocellular carcinoma cell genome cDNA.

References