Composite

Part:BBa_K1321366:Design

Designed by: Gabriella Santosa   Group: iGEM14_Imperial   (2014-10-09)
Revision as of 23:41, 21 October 2014 by Gabi1234 (Talk | contribs) (→‎Source)

CBDcex fused to sfGFP with T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 53
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 401


Design Notes

This part was made from the composite parts that we submitted, and fused by Biobrick and Freiburg cloning. SfGFP was fused as a reporter for our CBD binding assay, and may have uses for future teams as an easily quantifiable reporter.

Source

CBDcex comes from Cellulomonas fimi, and sfGFP is a modified super-folding mutant of GFP. Our sfGFP part was an improvement of BBa_I746909, wherein Freiburg sites were added. Please see BBa_K1321337 for more info on this part. CBDcex was requested from the registry, as it was submitted by the Freiburg team as BBa_K863101.

The T7 vector was made by inverse PCR of BBa_I746909, and ligated. Please see our BBa_ K1321338 for more information on this part.

References