Part:BBa_K1529310
Prhl(LR)
We newly developed the Prhl(LR) promoter (BBa_K1529310) by improving Prhl promoter (BBa_R0071) which is activated by C4HSL-RhlR complex (Fig. 1).
To characterize the function of this Prhl(LR) promoter, we constructed a part, Prhl(LR)-GFP (BBa_K1529311), by inserting the Prhl(LR) promoter, upstream of a GFP coding sequence.
By using the reporter cells that contain Prhl(LR)-GFP, we measured the fluorescence intensity of the cells induced by C4HSL. (Fig. 3.)
We saw that our new Prhl(LR) promoter was actually activated by C4HSL through an induction assay
For more information, see [http://2014.igem.org/Team:Tokyo_Tech/Experiment/Prhl_reporter_assay our work in Tokyo_Tech 2014 wiki].
iGEM 2016 Tokyo_Tech
We improve BBa_K1529310 and design BBa_K1949060.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
None |