Generator
Part:BBa_K1403003:Design
Designed by: Sylvia Group: iGEM14_Paris_Bettencourt (2014-10-06)
Limonene synthase (LIMS1) expression cassette
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 82
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 187
Design Notes
The purpose of this part is to produce limonene in E. coli.
We used LIMS1 CDS from part BBa_I742110 and inserted constitutive promoter BBa_J23108 and a sRBS upstream of the CDS. Limonene synthase 1 converts farnesyl-diphosphate to (+)-limonene, which is a component of lemon scent.
The promoter and RBS were designed as oligos, aligned, digested and ligated. The finished plasmid is in standard biobricks format.
Source
Registry. BBa_I742110
References
iGEM07_Edinburgh: https://parts.igem.org/Part:BBa_I742110
Salis Lab. RBS calculator: https://salis.psu.edu/software/forward H.M Salis, Methods in Enzymology 2011 H.M. Salis, E.A. Mirsky, C.A. Voigt, Nat. Biotech., 2009