Composite

Part:BBa_K1493501

Designed by: Michiel Herpers   Group: iGEM14_Wageningen_UR   (2014-10-06)
Revision as of 13:54, 21 October 2014 by M Herpers (Talk | contribs)

pRha + GFP

GFP controlled by pRha.

This BioBrick is used by [http://2014.igem.org/Team:Wageningen_UR BananaGuard] as a control in the [http://2014.igem.org/Team:Wageningen_UR/project/characterization rhamnose mediated characterization].

Wageningen_UR_killswitch_rhamnose.png

Figure 1. Average GFP fluorescence of E. coli DH5α carrying a pSB3K3 plasmid containing the pRha gfp BioBrick. At t=0 the cells were induced with different concentrations of rhamnose in triplo. Fluorescence is measured in a plate reader in time (A) and at time point 8.25 (B) normalized for OD600.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 792


[edit]
Categories
Parameters
None