Coding

Part:BBa_K1491017

Designed by: Danielle Peters   Group: iGEM14_Carnegie_Mellon   (2014-08-04)
Revision as of 16:02, 26 October 2017 by Chenhong (Talk | contribs)

Codon Optimized Supernova

Supernova is the monomeric version of KillerRed (BBa_K1184000). It has been codon optimized for E. coli.

Of the three superoxide generators, Supernova had the biggest decrease in viability following photobleaching when compared to its control. It also had the largest decrease in fluorescence following photobleaching. Fluorescence was measured at (ex/em = 585nm/610nm).

389px-LacSuperoxides.jpg
386px-ConstitutiveSuperoxides.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 50
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


improvement

2017 SSTi-SZGD Team has improved the this part,The improvement was made by inserting supernova gene into a light-regulated expression system, lightoff (BBa_K2244009), cell apoptosis can be induced by firstly expressing supernova gene in darkness, and then expose cells to light illumination that results to ROS release and the subsequent cell death. The entire process is purely regulated by light. We believe this has improved the original use of supernova in a T7 system using IPTG induction, which requires additional chemical inducer.
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Parameters
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