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Part:BBa_K1465103
Fumarate reductase subunit A (FumA) from Actinobacillus succinogenes
Fumarate reductase (FumABCD) from Actinobacillus succinogenes
Usage and Biology
Fumarate reductase is part of the anaerobic fumarate respiration in bacteria. It catalyzes the reaction from fumarate into succinate using fumarate as a final electron acceptor in anaerobic fumarate respiration. The related enzyme in aerobic respiration is the succinate dehydrogenase, which catalyses the reaction from succinate to fumarate.
The electrons are transferred from succinate to FAD+ producing fumarate and FADH2. The succinate dehydrogenase is also a membrane enzyme and it is part of the citric acid cycle. They both belong to the respiration complex II. Naturally there is no activity of both enzymes at the same time in E. coli cells. (Iverson et al., 1999)
In our project we used the fumarate reductase in combination with an extracellular mediator as electron donor to transfer electrons into bacterial cells. The reduced mediator crosses the outer membrane of E. coli through the outer membrane porine OprF (BBa_K1172507).
Mediators adsorb at the inner membrane and transfer electrons to the fumarate reductase. After that the reduced fumarate reductase transfer electrons to fumarate producing succinate. This process has been shown for the fumarate reductase in Actinobacillus succinogenes by Park et al..
As published by Park et al., 1999 the fumarate reductase from A. succinogenes shows high activity accepting electrical reduced neutral red as an electron donor and so stimulates the metabolism in a electrobiochemical reactor system. We planed to compare activity of fumarate reductase Fum of A. succinogenes with fumarate reductase Frd of E. coli (a href="https://parts.igem.org/Part:BBa_K1465102">BBa_K1465102) with regard to activity accepting neutral red or bromphenol blue as alternative electron acceptors in our electrobiochemical reactor system.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 734
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1120
Results
References
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Iverson et al., 1999. Structure of the Escherichia coli Fumarate Reductase Respiratory Complex. Science, vol. 284, pp. 1961-1966
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Park et al., 1999. Utilization of Electrically Reduced Neutral Red byActinobacillus succinogenes: Physiological Function of Neutral Red in Membrane-Driven Fumarate Reduction and Energy Conservation. Journal of Bacteriology, vol. 181, pp. 2403-2410
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