Plasmid

Part:BBa_K1391101:Design

Designed by: Shinjini Saha   Group: iGEM14_MIT   (2014-10-17)
Revision as of 18:58, 20 October 2014 by Clrichar (Talk | contribs) (→‎Design Notes)


pEXPR_Gantenerumab Variable Human Ig-M Light Chain


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 532
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 532
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 532
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 532
    Illegal AgeI site found at 64
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Gantenerumab has shown therapeutic potential in recent clinical trials. Human Ig-M can be anchored to the membrane to interact with the b-cell receptor (BCR) complex. Human antibody membrane localization tag is natively found in human, membrane-bound antibodies.

This gene is expressed under a hEF1a promoter for constitutive high level expression in mammalian cells. It adheres to RFC 65 for mammalian parts. (Ignore the RFC 65 link it is an automated link that links to a page unrelated to the iGEM RFC 65)

Source

Fusion protein of 3 human peptide sequences


References