Plasmid

Part:BBa_K1391132:Design

Designed by: Shinjini Saha   Group: iGEM14_MIT   (2014-10-17)
Revision as of 16:20, 30 October 2014 by Clrichar (Talk | contribs) (→‎Design Notes)


pEXPR_TRE:miRNAG3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3087
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3087
    Illegal NheI site found at 2124
    Illegal NheI site found at 2390
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3087
    Illegal BamHI site found at 2554
    Illegal XhoI site found at 2984
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3087
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3087
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3609
    Illegal SapI.rc site found at 2001
    Illegal SapI.rc site found at 2602


Design Notes

This part was created using a single pot LR reaction. The promoter is flanked by B4 and P1 sites and the gene is flanked by P1 and B2 sites. Either of these can be extracted using a BP reaction. This part adheres to RFC 65 for recombination based cloning of mammalian parts.

Source

Artificial

References