Plasmid

Part:BBa_K1391129:Design

Designed by: Shinjini Saha   Group: iGEM14_MIT   (2014-10-17)
Revision as of 16:18, 30 October 2014 by Clrichar (Talk | contribs) (→‎Design Notes)

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pEXPR_BACE2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 4598
    Illegal EcoRI site found at 5641
    Illegal EcoRI site found at 5688
    Illegal EcoRI site found at 6237
    Illegal XbaI site found at 4525
    Illegal PstI site found at 2677
    Illegal PstI site found at 3656
    Illegal PstI site found at 3939
    Illegal PstI site found at 4900
    Illegal PstI site found at 5523
    Illegal PstI site found at 5966
    Illegal PstI site found at 6774
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 4598
    Illegal EcoRI site found at 5641
    Illegal EcoRI site found at 5688
    Illegal EcoRI site found at 6237
    Illegal NheI site found at 3210
    Illegal PstI site found at 2677
    Illegal PstI site found at 3656
    Illegal PstI site found at 3939
    Illegal PstI site found at 4900
    Illegal PstI site found at 5523
    Illegal PstI site found at 5966
    Illegal PstI site found at 6774
    Illegal NotI site found at 7079
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 4598
    Illegal EcoRI site found at 5641
    Illegal EcoRI site found at 5688
    Illegal EcoRI site found at 6237
    Illegal BglII site found at 6317
    Illegal BamHI site found at 2854
    Illegal BamHI site found at 3328
    Illegal BamHI site found at 7274
    Illegal XhoI site found at 3538
    Illegal XhoI site found at 7059
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 4598
    Illegal EcoRI site found at 5641
    Illegal EcoRI site found at 5688
    Illegal EcoRI site found at 6237
    Illegal XbaI site found at 4525
    Illegal PstI site found at 2677
    Illegal PstI site found at 3656
    Illegal PstI site found at 3939
    Illegal PstI site found at 4900
    Illegal PstI site found at 5523
    Illegal PstI site found at 5966
    Illegal PstI site found at 6774
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 4598
    Illegal EcoRI site found at 5641
    Illegal EcoRI site found at 5688
    Illegal EcoRI site found at 6237
    Illegal XbaI site found at 4525
    Illegal PstI site found at 2677
    Illegal PstI site found at 3656
    Illegal PstI site found at 3939
    Illegal PstI site found at 4900
    Illegal PstI site found at 5523
    Illegal PstI site found at 5966
    Illegal PstI site found at 6774
    Illegal NgoMIV site found at 2297
    Illegal NgoMIV site found at 3741
    Illegal NgoMIV site found at 4024
    Illegal NgoMIV site found at 5353
    Illegal NgoMIV site found at 5809
    Illegal AgeI site found at 3456
    Illegal AgeI site found at 4638
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1121
    Illegal BsaI.rc site found at 5284
    Illegal SapI site found at 38
    Illegal SapI site found at 3214
    Illegal SapI site found at 7506


Design Notes

This part was created using a single pot LR reaction. The promoter is flanked by B4 and P1 sites and the gene is flanked by P1 and B2 sites. Either of these can be extracted using a BP reaction. This part adheres to RFC 65 for recombination based cloning of mammalian parts.

Source

Human

References