Regulatory

Part:BBa_K1509003

Designed by: Tong Li   Group: iGEM14_NEFU_China   (2014-09-26)
Revision as of 02:09, 18 October 2014 by LiTong (Talk | contribs) (→‎Result)

CP25 is a highly active,constitutive promoter

The synthetic CP25 promoter was designed to be constitutive. CP25 is a stronge promoter in Lactococcus lactis by Jensen and Hammer.In the article,they measure the activities of the CP promoter in E. coli.The promoter activities were assayed from the expression of a reporter gene (lacLM) encoding β-galactosidase transcribed from the different synthetic promoter clones on the promoter cloning vector pAK80.The result suggests CP25 is the strongest promoter.

Usage and Biology

File:Nanocrystal.png


fig1.CDS7 gene was under control of CP25 promoter.


Result

Nanocrystal3.png


fig2.The E.coli in the feild of TEM.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//promoter
Parameters
None