Coding

Part:BBa_K1336003:Design

Designed by: Yan-Kay Ho   Group: iGEM14_UCL   (2014-10-06)
Revision as of 01:08, 18 October 2014 by Ddelatorre (Talk | contribs) (→‎Design Notes)

Dye-decolourising peroxidase from Bacillus subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 97
    Illegal BsaI site found at 106
    Illegal BsaI site found at 1072
    Illegal SapI site found at 502
    Illegal SapI site found at 860
    Illegal SapI.rc site found at 1016


Design Notes

PCR amplified with prefix and suffix. Primers used:

PRE BsDyPF4 : 5’ - GTT TCT TCG AAT TCG CGG CCG CTT CTA G ATG AGC GAT GAA CAG AAA AAG - 3’
SUF REV BsDyP : 5’ - GT TTC TTC CTG CAG CGG CCG CTA CTA GTA TTA TTA TGA TTC CAG CAA ACG CTG GG - 3’

Source

From genomic sequence of Bacillus subtilis; BsDyP gene inserted into a pET-21a (+) expression vector, provided by the Microbial & Enzyme Technology Lab Research Group, from Instituto de Tecnologia Química e Biológica in Lisbon University.

References