RNA

Part:BBa_K1323008:Design

Designed by: Julia Manalil   Group: iGEM14_Waterloo   (2014-10-07)
Revision as of 20:45, 17 October 2014 by Rkovac31 (Talk | contribs)

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sRNA control for S. epidermidis: targets the non-coding region


This is a control sRNA that targets a non-coding region in the genome of S.epidermidis (2314773-2314796) which is a intergenic region that is downstream of two genes on two different strands. It contains binding energy of only -7.8 kcal/mol to YFP gene as determined using DINAMelt software (http://mfold.rna.albany.edu/?q=DINAMelt/Two-state-melting). For effective binding, sRNA must have binding energy between -30 kcal/mol to -40 kcal/mol (Yoo et al., 2013). This shows that control sRNA cannot effectively bind YFP and it serves as a control which allows us to determine if production of sRNA places a metabolic load on the organism (Dokyun et al., 2013).



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 138
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]