Composite

Part:BBa_K1413024:Design

Designed by: Laura MATABISHI, Nandjafot MENDY   Group: iGEM14_Evry   (2014-10-17)
Revision as of 21:56, 1 November 2014 by Laurabibi (Talk | contribs) (→‎References)

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bphR1-RBS-RFP-Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 156
    Illegal BamHI site found at 239
    Illegal XhoI site found at 46
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 883
    Illegal AgeI site found at 995
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 139


Design Notes

Between each part, there is the scar SpeI/XbaI


Source

All the parts come from the registry

References

Kensuke Furukawa and Hidehiko Fujihara, Microbial Degradation of Polychlorinated Biphenyls : Biochemical and Molecular Features, 2008. Journal of Bioscience and Bioengineering, Vol. 105, No. 5, 433–449.