Part:BBa_K1486049

CpxR promoter FW + RFP

The objective of this BioBrick is to improve the stress sensor of the BBa_K1486048 [1]. So the regulatory sequence was replaced with the genomic sequence of the E.coli genome (the regulatory sequence of CpxA with the CpxR consensus sequence). Because this regulatory sequence is suspected to regulate in both directions it was also placed in front of RFP the other way around (BBa_K1486050).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 727
  Illegal AgeI site found at 839
  
• 1000
  COMPATIBLE WITH RFC[1000]

Characterisation

To check if the regulatory sequence in front of the RFP coding sequence is sensible to chemical stress we checked it in 80 mM KCl solution in direct comparison with the BBa_K1486048 and the BBa_K1486050. The same cells where treated with PBS as a negative control. The positive control is not shown in the graphe because its light emission is 3 orders of magnitude bigger.

Usage

Excitation: 555 nm Emission: 632 nm