Generator

Part:BBa_K1486008

Designed by: iGem EPFL 2014   Group: iGEM14_EPF_Lausanne   (2014-08-26)
Revision as of 15:34, 17 October 2014 by Robert Baldwin (Talk | contribs)

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CxpR & Split IFP1.4 [Cterm + Cterm][1]

Usage and Biology

This biobricked was an intermediate for BBa_K1486056.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2213
    Illegal PstI site found at 3446
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal PstI site found at 2213
    Illegal PstI site found at 3446
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3657
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 1283
    Illegal XhoI site found at 2470
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2213
    Illegal PstI site found at 3446
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2213
    Illegal PstI site found at 3446
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1694
    Illegal AgeI site found at 2881
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
//cds/reporter
//cds/transcriptionalregulator
//cds/transcriptionalregulator/activator
//chassis/prokaryote/ecoli
//classic/regulatory/other
//direction/forward
//function/regulation/transcriptional
//function/reporter/fluorescence
//promoter
//rbs/prokaryote/constitutive
//regulation/multiple
regulator
transcriptional
Parameters
biology
chassisEscherichia coli
colorInfrared
controlAraC, arabinose
directionForward
emission640
excitation710
functionTransciption regulator
negative_regulatorsCpxA (phosphatase activity)
positive_regulatorsCpxA (Histidine kinase activity when activated)
rbsElowitz
resistanceChloramphenicol