Coding

Part:BBa_K1509002:Design

Designed by: Tong Li   Group: iGEM14_NEFU_China   (2014-09-26)
Revision as of 01:20, 18 October 2014 by LiTong (Talk | contribs) (→‎Design Notes)

Design Notes

It was attained from Bacillus sp. F2 by using PCR with the total genomic DNA of F2 as template. The two synthesized oligonucleotide primers were shown as below:

P1: 5’-AGTCTAGATGTAAGCTCTCTTCCGG-3’

P2:5’-CTCTCGAGCAATAAGGACGCAATG-3’ And the PCR runs under the settings as follows:

Flocculation PCR.png

Then it was spliced with the DNA sequence for peptide CDS 7 with a synthetic constitutive promoters CP25.

Sourse

It’s origin is one strain named as Bacillus sp. F2, whose flocculent ability can reach 84%.