Generator

Part:BBa_K1486008:Design

Designed by: iGem EPFL 2014   Group: iGEM14_EPF_Lausanne   (2014-08-26)
Revision as of 14:20, 17 October 2014 by Darth Vader (Talk | contribs) (→‎Source)

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CxpR & Split IFP1.4 [Cterm + Cterm][1]


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2213
    Illegal PstI site found at 3446
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal PstI site found at 2213
    Illegal PstI site found at 3446
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3657
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 1283
    Illegal XhoI site found at 2470
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2213
    Illegal PstI site found at 3446
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2213
    Illegal PstI site found at 3446
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1694
    Illegal AgeI site found at 2881
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

It is to be noted that this construct makes use of an Arabinose-inducible promoter, a standard Elowitz RBS, as well as a flexible linker 2 x (Gly-Gly-Gly-Gly-Ser) between CpxR and the two split parts of the IFP. WARNING: It is also to be noted that we had to submit basic parts with a start codon and a stop codon, which may have been removed to make the composites part, so that only the first protein cds have a START codon and only the last protein cds have a STOP codon. You should consider it when looking at the part sequence.

Source

We received Michnick lab IFP1 and IFP2 sequences from addgene.

References

Michnick, S., Tchekanda, E., & Sivanesan, D. (2014, April 20). [http://www.nature.com/nmeth/journal/v11/n6/full/nmeth.2934.html An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions.] Nature Methods, 6-6.