Coding

Part:BBa_K1509002:Design

Designed by: Tong Li   Group: iGEM14_NEFU_China   (2014-09-26)
Revision as of 14:17, 17 October 2014 by LiTong (Talk | contribs) (→‎Design Notes)

Design Notes

It was attained from Bacillus sp. F2 by using PCR with the total genomic DNA of F2 as template. The two synthesized oligonucleotide primers were shown as below: P1: 5’-AGTCTAGATGTAAGCTCTCTTCCGG-3’ P2:5’-CTCTCGAGCAATAAGGACGCAATG-3’ And the PCR runs under the settings as follows:

Temperature： 96℃ 94℃ 50℃ 72℃ 72℃

Time： 15min 40sec 60sec 4min 7min

Number of cycle： 1 30 30 30 1

Then it was spliced with the DNA sequence for peptide CDS 7 with a synthetic constitutive promoters CP25.

Sourse

It’s origin is one strain named as Bacillus sp. F2, whose flocculent ability can reach 84%.