Coding
Part:BBa_K1509002:Design
Designed by: Tong Li Group: iGEM14_NEFU_China (2014-09-26)
Design Notes
It was attained from Bacillus sp. F2 by using PCR with the total genomic DNA of F2 as template. The two synthesized oligonucleotide primers were shown as below: P1: 5’-AGTCTAGATGTAAGCTCTCTTCCGG-3’ P2:5’-CTCTCGAGCAATAAGGACGCAATG-3’ And the PCR runs under the settings as follows:
Temperature： 96℃ 94℃ 50℃ 72℃ 72℃
Time： 15min 40sec 60sec 4min 7min
Number of cycle： 1 30 1
Then it was spliced with the DNA sequence for peptide CDS 7 with a synthetic constitutive promoters CP25.
Sourse
It’s origin is one strain named as Bacillus sp. F2, whose flocculent ability can reach 84%.