Coding
BFR

Part:BBa_K1438000:Design

Designed by: Johann Bauerfeind   Group: iGEM14_Berlin   (2014-09-13)
Revision as of 08:52, 17 October 2014 by JohannB (Talk | contribs) (→‎References)

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Bacterioferritin (BFR)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 34
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 142


Design Notes

The featured N-terminal His-tag makes it easy for all future iGEM teams to purify the construct using Ni-NTA affinity chromatography.

For any metal nanoparticles built inside of BFR it would be beneficary to remove any heme groups in order to prevent unloading of BFR by heme-mediated electron transfer. We constructed such a improved biobrick BBa_K1438001

Source

Bacterioferritin from E. coli Nissle 1917 genomic DNA. Amplified by using PCR and cloned into pQE80L

References

[1] Frolow F, Kalb AJ, Yariv J. Structure of a unique twofold symmetric haem-binding site. Nat Struct Biol. 1994 Jul;1(7):453-60. PubMed PMID: 7664064.

[2] Yao H, Wang Y, Lovell S, Kumar R, Ruvinsky AM, Battaile KP, Vakser IA, Rivera M. The structure of the BfrB-Bfd complex reveals protein-protein interactions enabling iron release from bacterioferritin. J Am Chem Soc. 2012 Aug 15;134(32):13470-81. doi: 10.1021/ja305180n. Epub 2012 Aug 1. PubMed PMID: 22812654; PubMed Central PMCID: PMC3428730.

[3] Grosse C, Scherer J, Koch D, Otto M, Taudte N, Grass G. A new ferrous iron-uptake transporter, EfeU (YcdN), from Escherichia coli. Mol Microbiol. 2006 Oct;62(1):120-31. PubMed PMID: 16987175.

[4] Hill PJ, Stritzker J, Scadeng M, Geissinger U, Haddad D, Basse-Lüsebrink TC, Gbureck U, Jakob P, Szalay AA. Magnetic resonance imaging of tumors colonized with bacterial ferritin-expressing Escherichia coli. PLoS One. 2011;6(10):e25409. doi: 10.1371/journal.pone.0025409. Epub 2011 Oct 3. PubMed PMID: 21984917; PubMed Central PMCID: PMC3184983.

[5] Hawkins C, William J.M Mössbauer studies of superparamagnetism in E. coli.i. Journal of Magnetism and Magnetic Materials 104-107 (1992) 1549-1550 North-Holland Sequence and Features