Plasmid

Part:BBa_K1391010

Designed by: Shinjini Saha   Group: iGEM14_MIT   (2014-10-17)
Revision as of 02:12, 31 October 2014 by Shinjini37 (Talk | contribs)

pENTR_PirB

PirB (paired immunoglobin-like receptor B) is a transmembrane protein found in murine neurons, shown to bind to beta-amyloid. The interactions is mediated by the first two extracellular immunoglobulin (Ig) domains. Upon binding to beta-amyloid (extracellular), PirB induces enhanced cofilin signaling (intracellular).

Taeho Kim, George S. Vidal, Maya Djurisic, et al. "Human LilrB2 is a beta-amyloid recptor and its murine homolog PirB regulates synaptic plasticity in an Alzheimer's Model". Science. 20 Sep 2013. <http://www.sciencemag.org/content/341/6152/1399>. 16 Oct 2014.

The characterization of the system this is part of can be found in the experience page.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 244
    Illegal EcoRI site found at 1735
    Illegal PstI site found at 1324
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 244
    Illegal EcoRI site found at 1735
    Illegal PstI site found at 1324
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 244
    Illegal EcoRI site found at 1735
    Illegal BamHI site found at 53
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 244
    Illegal EcoRI site found at 1735
    Illegal PstI site found at 1324
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 244
    Illegal EcoRI site found at 1735
    Illegal PstI site found at 1324
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2062


[edit]
Categories
Parameters
None