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Coding

Part:BBa_K1323011

Designed by: Julia Manalil   Group: iGEM14_Waterloo   (2014-10-04)
Revision as of 15:10, 17 October 2014 by Rkovac31 (Talk | contribs)

Erythromycin resistance coding sequence

This coding sequence is for the antibiotic, erythromycin. This part is PCR amplified from ermM cassette synthesized by Biobasic. This ermM cassette is originally found in S.epidermidis plasmid pNE131 which confers resistance to macrolide-lincosamide-streptogramin B group antibiotics including erythromycin (Catchpole et al., 1988). Working concentration for this antibiotic is 10 µg/mL in S.epidermidis/S.aureus hosts.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Catchpole, I., Davies, T. A., and Dyke, K. G. H. (1988). The nucleotide sequence of Staphylococcus aureus plasmid pT48 conferring inducible macrolide-lincosamide-streptogramin B resistance and comparison with similar plasmids expressing constitutive resistance. Journal of General Microbiology 134, 697-709.


[edit]
Categories
//cds/selectionmarker/antibioticresistance
Parameters
None