Coding

Part:BBa_K1431101

Designed by: Rifei Chen, Yushan Zhang   Group: iGEM14_SUSTC-Shenzhen   (2014-10-09)
Revision as of 05:11, 17 October 2014 by ChenRifei (Talk | contribs)

TetOn-3G, an ideal controller of mammalian gene expression with TRE-3G promoter+PolyA

TetOn(Tetracycline-Controlled Transcriptional Activation,also known as rtTA2S-M2) is a system of inducible gene expression systems for mammalian cells. TetOn-3G (also known as rtTA-V16[citation needed]) is similar to TetOn but was derived from rtTA2S-S2 rather than rtTA2S-M2. The TetOn-3G protein has 5 amino acid differences compared to TetOn which appear to increase its sensitivity to Dox even further. TetOn-3G is sensitive to 100-fold less Dox and is 7-fold more active than the original TetOn.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None