Part:BBa_J34805
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terminator |
Secondary Structure
Measurement
- [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
Contribution: New documentation from Evry_Paris-Saclay 2020
BBa_B0015 is a composite terminator made by joining 2 other terminators, one derived from E. coli (BBa_B0010) and the other from the T7 phage (BBa_B0012). Unlike what one can guess from the name and origin, the E. coli terminator can terminate transcription by T7 RNA polymerase, but not the T7 TE terminator. Indeed, BBa_B0010 is the E. coli rrnB T1 terminator which was shown to be an efficient terminator for the E. coli RNA polymerase, but also for the phage SP6 and T7 RNA polymerases through two different mechanism: one involving an upstream hairpin structure and the other one a downstream sequence-specific signal [1–3]. However, the T7 TE terminator (BBa_B0012) is located at the end of the T7 DNA ligase gene which is in the early region of bacteriophage T7 genome [4]. This terminator is an efficient one for the E. coli RNA polymerase, but not for the T7 RNA polymerase [5].
References
[1] Christiansen J. The 9S RNA precursor of Escherichia coli 5S RNA has three structural domains: implications for processing. Nucleic Acids Research (1988) 16: 7457–7476.
[2] Hartvig L, Christiansen J. Intrinsic termination of T7 RNA polymerase mediated by either RNA or DNA. The EMBO journal (1996) 15: 4767–4774.
[3] Kwon YS, Kang C. Bipartite modular structure of intrinsic, RNA hairpin-independent termination signal for phage RNA polymerases. The Journal of Biological Chemistry (1999) 274: 29149–29155.
[4] Dunn JJ, Studier FW. Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements. Journal of Molecular Biology (1983) 166: 477–535.
[5] Dunn JJ, Studier FW. The transcription termination site at the end of the early region of bacteriophage T7 DNA. Nucleic Acids Research (1980) 8: 2119–2132.
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