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Applications of BBa_K537020
User Reviews
UNIQ8dc424406f8e0d3b-partinfo-00000000-QINU
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HZAU-China 2014
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We want to know whether recombination will happen without the cre recombinase. So we design the device to verify. We only transform this device[fig.1] into DH5α and DH10B.At the same time, the samples were sequenced and anything was OK. In summary, we confirmed the unexpected recombination of lox71 site even if the device is in the stable E.coli strains such as the DH5α and DH10B. But the probability of its occurrence is extremely low. And it doesn’t affect the sequencing result in a short time. But as we explained in the tetR repressor protein (BBa_C0040), we don’t recommend you used the cre/lox system to achieve the toxic protein’s deletion or rolling over.
 fig.1:Confirmation Device |
UNIQ8dc424406f8e0d3b-partinfo-00000002-QINU
