Signalling

Part:BBa_K1541017:Design

Designed by: Daniel Gerngross   Group: iGEM14_ETH_Zurich   (2014-10-06)
Revision as of 14:20, 26 October 2014 by Mbahls (Talk | contribs) (→‎Source)

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rhlI with optimized RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The [http://salis.psu.edu/software/RBSLibraryCalculatorSearchMode RBS Calculater] provided by the Salis Lab was used to find an optimized RBS for the genetic context of rhlI. The calculator provides a value for the translation initiation rate (TIR), which was shown to highly correlate with protein expression levels[1].

Source

References

[1] [http://www.nature.com/nbt/journal/v27/n10/full/nbt.1568.html Salis, H. M., Mirsky, E. A., Voigt, C. A., Automated design of synthetic ribosome binding sites to control protein expression, Nature Biotechnology, 27, 2009]