Generator

Part:BBa_K1418061

Designed by: Ryan Putman   Group: iGEM14_Utah_State   (2014-10-16)
Revision as of 03:56, 18 October 2014 by Ryanputman (Talk | contribs)

phaCAB-p-GFPPhaC-B0015

Inducible PHB generator with GFP fused to PhaC (PHB synthase).

Phbgranule2.png

Schematic for functionalization of PHB granules. PhaC remains covalently bound to the PHB granule after polymerization and GFP can be fused to the N-terminal of PhaC, thus functionalizing the PHB granule. Direct production of PHB with the phaABC operon and expression of the GFP-PhaC fusion protein are both under lactose control.

USU2014_NileBlueStaning.PNG

A.) Nile Blue staining and B.) GFP expression of E. coli cells containing <a href="https://parts.igem.org/Part:BBa_K1418061"> BBa_K1418061</a>. Microscope images of GFP bound PHB granules in E. coli after 24 h of growth with IPTG induction. White bar represents 5 µm.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4446
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 916
    Illegal BglII site found at 1741
    Illegal BglII site found at 5761
    Illegal BamHI site found at 4965
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 222
    Illegal NgoMIV site found at 293
    Illegal NgoMIV site found at 893
    Illegal NgoMIV site found at 1205
    Illegal NgoMIV site found at 1484
    Illegal NgoMIV site found at 2136
    Illegal NgoMIV site found at 2158
    Illegal AgeI site found at 5460
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4002


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Parameters
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