Composite

Part:BBa_K1480002

Designed by: Jesus Gilberto Rodriguez Ceja   Group: iGEM14_UANL_Mty-Mexico   (2014-10-08)
Revision as of 00:38, 17 October 2014 by GilbertoRdz (Talk | contribs) (→‎Results)

This part is regulated by the promoter ptet (R0040) and codifies RFP/mCherry protein generator (K081014). It was constructed using 10 assembly.

Usage

It is a reporter protein that switch off when the repressor TetR binds to ptet.

Results

It is shown in red the relative fluorescence of clones without repressor. The blue bars are for the same clones regulated by the TetR generator K145201.The mean and standard deviation for red bars are 4045 and 226 and for the blue bars are 128 and 2500.
[edit]
Categories
//cds/reporter/rfp
//classic/reporter
//direction/forward
Parameters
colorRed
directionForward
emission607
excitation584
functionReporter
negative_regulatorsregulated by TetR
proteinmRFP