Coding

Part:BBa_K1342002:Design

Designed by: Naoki Saito,Daiki Haraguchi,Yoshiharu Otaki,Ryuhei Minei   Group: iGEM14_Nagahama   (2014-10-03)
Revision as of 19:35, 16 October 2014 by Naoki (Talk | contribs) (→‎Source)


AspA LVA(-)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 72
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 964
    Illegal AgeI site found at 493
    Illegal AgeI site found at 1345
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1004


Design Notes

soon


Source

  • EcoCyc[http://ecocyc.org/ECOLI/NEW-IMAGE?type=OPERON&object=TU0-8330 ZinT] [http://ecocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10095 AspA]
  • E. coli JM109

References

  • Miroslaw Papierz. Selection and Activation of Escherichia coli Strains for L-aspartic Acid Biosynthesis. polish journal of microbiology 2007, Vol.56, No2, 71-76
  • Grazyna Gadomska. Recombinant Strains of Escherichia coli for L-aspartic Acid Biosynthsis. polish journal of microbiology 2007, Vol.56, No2, 77-82.
  • A. Puskarova. Regulation of yodA encoding a novel cadmium-induction protein in Escherichia coli. Microbiology 2002, 148, 3801-3811