Composite

Part:BBa_K1326004

Designed by: Leah Simmons   Group: iGEM14_Macquarie_Australia   (2014-10-08)
Revision as of 23:55, 17 October 2014 by Leah.Simmons (Talk | contribs) (→‎Functional Parameters)

Plac + ChlI1 + ChlD

Composite part incorporating the genes adding a Mg ion to protoporphyrin IX, as a part of the chlorophyll biosynthetic pathway. Magnesium chelatase subunit I (ChlI1) and Magnesium chelatase subunit D (ChlD) form an ATP-dependent complex (increased yield is seen if ChlI2 subunit is also present). This complex acts on protoporphyrin IX, bound to the ChlH protein, and inserts a magnesium ion which produces Mg-protoporphyrin IX.

Has since been added to: BBa_K1326008


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 2666
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 439
    Illegal BglII site found at 1095
    Illegal BglII site found at 1290
    Illegal BglII site found at 3371
    Illegal BamHI site found at 487
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1946
    Illegal NgoMIV site found at 2765
    Illegal NgoMIV site found at 3307
  • 1000
    COMPATIBLE WITH RFC[1000]



Functional Parameters


Fluorescence Spectra of ChlI1 + ChlD at 420nm Excitation.png
Figure 1: Fluorescence spectra at 420 nm excitation of induced ChlI1+ChlD extract following incubation in Figure 1. Peak observed at 595nm is consistent with Mg-Protoporphyrin IX production, demonstrating significant activity of the submitted BioBrick part.
Fluorescence Intensity of Mg-Protoporphyrin Formation.png
Figure 2: Increasing fluorescence intensity over time through function of /ChlI1 + ChlD/ composite part..

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Parameters
None