Device

Part:BBa_K1373001:Design

Designed by: Wenxuan Chen   Group: iGEM14_SCAU-China   (2014-09-23)
Revision as of 05:49, 16 October 2014 by Scau cwx (Talk | contribs) (→‎Design Notes)

nadE with strong promter and strong RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

cloning via omega-PCR

Primers

F-nadE:ctagctactagagaaagaggagaaaATGACATTGCAACAACAAATAATAAAG

R-nadE:GATGATTTCTGGAAAAAGTAAtactagtagcggccgctgcagtc

Source

the promoter is from iGEM distribution kit and the nadE gene is from the genome of E.coli MG1655

References