![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K1462090:Design
Designed by: Chuyao Fan Group: iGEM14_SCUT (2014-10-10)
Gal1+cox6+GFP+ADH1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 150
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1325
Design Notes
To test the localization function of CoxVI, the C-terminus of CoxVI was fused to GFP. Besides,we use MitoTracker Red®CMXRos to stain the mitochondria and do an overlap with GFP. By observing the coefficient value, we can judge that if our leading peptide works.
Source
COXVI is obtained from genome of saccharomyces cerevisiae, the other parts is from the kit provided by official