Device

Part:BBa_K1475010:Design

Designed by: Daniel Weltz Pedersen   Group: iGEM14_SDU-Denmark   (2014-10-07)
Revision as of 20:08, 15 October 2014 by Danie12 (Talk | contribs) (→‎Source)

dxs+LIMS1+appY regulated by the tet promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 808
    Illegal SapI.rc site found at 2068


Design Notes

The part was constructed using standard assembly from: Part: BBa_K1475003 and Part:BBa_K118024


Source

Dxs: Escherichia coli
LIMS1: Citrus lemon
appY: Escherichia coli

References

1. Leighanne A. Brammer et al.: 1-Deoxy-d-xylulose 5-Phosphate Synthase Catalyzes a Novel Random Sequential Mechanism. J Biol Chem. Oct 21, 2011; 286(42): p. 36522–36531. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3196101/
2. BRENDA, 2014: EC 2.2.1.7 - 1-deoxy-D-xylulose-5-phosphate synthase. http://www.brenda-enzymes.org/enzyme.php?ecno=2.2.1.7&Suchword=&organism%5B%5D=Escherichia+coli&show_tm=0
3. BRENDA, 2014: EC 4.2.3.20 - (R)-limonene synthase. http://www.brenda-enzymes.org/enzyme.php?ecno=4.2.3.20&Suchword=&organism%5B%5D=Citrus+limon&show_tm=0
4. UniProt, P05052- APPY_ECOLI, http://www.uniprot.org/uniprot/P05052