Composite

Part:BBa_K1480003

Designed by: Jesus Gilberto Rodriguez Ceja   Group: iGEM14_UANL_Mty-Mexico   (2014-10-14)
Revision as of 04:45, 17 October 2014 by GilbertoRdz (Talk | contribs)

TetR generator (Promoter J23109)

This is a TetR protein generator regulated by the constitutive promoter J23109.

The protein has a LVA tag for degradation.

Results

It is shown the effect of 4 different TetR generators on a mCherry gene (K1140003) that is down regulated by ptet. To the right side are listed the names of the generators.

Here are the links for the other parts used.

K1140003 
K1480003 
k145201 
K1480004 
K1480005


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/transcriptionalregulator/repressor
//classic/generator/uncategorized
//direction/forward
//function/regulation/transcriptional
Parameters
directionForward
familyTetR
functionRepressor
ligandsATc / Tetracycline
proteinTetR repressor
tagLVA
targetptet