Part:BBa_K1537028

AHA2

Stomata are microscopic pores surrounded by two guard cells and play an important role in the uptake of CO2 for photosynthesis. Recent researches revealed that light-induced stomatal opening is mediated by at least three key components: blue light receptor phototropin, plasma membrane H+-ATPase, and plasma membrane inward-rectifying K+ channels.However, Yin Wang, et al[1]showed that only increasing the amount of H+-ATPase in guard cells had a significant effect on light-induced stomatal opening. Transgenic Arabidopsis plants by overexpressing H+-ATPase in guard cells exhibited enhanced photosynthesis activity and plant growth. Therefore,in order to improve the ability of absorbing formaldehyde, we overexpresse H+-ATPase(At AHA2) in transgenic tobacco guard cells ,resulting in a significant effect on light-induced stomatal opening(Yin Wanga et al,2014).

Fig. 1 (A) Typical fluorescence images of immunohistochemical detection of the guard cell H+-ATPase in the Arabidopsis epidermis. (B) qPCR analysis of AHA2 expression. Error bars represent the SEM (n ≥ 6). Significant differences were detected by Student t test (***P < 0.001). (C) Typical stomata in the epidermis illuminated with light for 30 min. References Yin Wanga,Ko Noguchib, Natsuko Onoa, Shin-ichiro Inouea, Ichiro Terashimab, and Toshinori Kinoshitaa,(2014)Overexpression of plasma membrane H+-ATPase in guard cells promotes light-induced stomatal opening and enhances plant growth. PNAS .111(1) .533–538

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]