Coding
Cel5A

Part:BBa_K1350007

Designed by: Yiting Luo   Group: iGEM14_SZU-China   (2014-09-28)
Revision as of 15:54, 13 October 2014 by Yitingluo (Talk | contribs) (→‎Usage and Biology)

Cel5A, endoglucanase（BBa_K805011 Improved）

we got this endoglucanase from iGEM kit,and we mutate it in a certain spot.[1]

Cel5A, endoglucanases

The endoglucanase genes cel5A can hydrolyze β-1,4glucosan randomly and act on long cellulose chains. The main product is oligomeric glucose. It can digest glucosan to produce glucose monomer in high efficiency with synergy with β-1,4glucosidase.It shows high ability to hydrolyze the crystal cellulose but relative lower enzyme activity towards carboxymethyl cellulose, barley β-glucan, and PASC.

Usage and Biology

Different from BBa_K805011 given by iGEM12_HUST-China, we have done the mutation of an ? to ?? and change the sequence in a way. However,because of the degeneracy of codon,the different sequence can encode the same enzyme.


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n/aCel5A, endoglucanase（BBa_K805011 Improved）