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Part:BBa_K1463000

Designed by: Beth Greig   Group: iGEM14_Glasgow   (2014-09-09)
Revision as of 12:54, 13 October 2014 by Cakesjason (Talk | contribs)

Recombinase Switch With GFP and RFP

A recombinase switch with GFP one side of the switch and RFP on the other.

[[|thumb|500px|right|Fig. 1: 1.pZJ7 on its own, 2.Switch #2 on its own, 3.Switch #2 + pZJ7 glucose, 4.Switch #2 + pZJ7 arabinose, 5.Switch #3 on its own, 6.Switch #3 + pZJ7 glucose, 7.Switch #3 + pZJ7 arabinose, 8.Switch #4 on its own, 9.Switch #4 + pZJ7 glucose, 10.Switch #4 + pZJ7 arabinose, 11.pBAD33 gvpAC (ignore)12.1kb+ marker.]]

GU_jake_gel_1.jpg

The image below shows a schematic of the switch. The



Switch_diagram_sites_small2.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 813
    Illegal NheI site found at 836
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 793
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 34
    Illegal AgeI site found at 146
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 711
    Illegal BsaI.rc site found at 1668


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