Composite

Part:BBa_K1366102

Designed by: Eduardo Cepeda Cańedo, Mercedes Alejandra Vazquez Cantu   Group: iGEM14_Tec-Monterrey   (2014-10-10)
Revision as of 02:24, 18 October 2014 by Eduardo250292 (Talk | contribs)

Genetic construct for msbB gene deletion (C2)

This construct contains the sequence homologies to delete the msbB gene (Braun’s lipoprotein) with a kanamycin resistance and the sequences for the Lux I production (AHL synthase). The deletion of msbB gene and the replacement of that sequence with the Kan resistance and LuxI will be performed with the red-lambda system. The FTR sequences included are used to remove the antibiotic resistance with a specific recombinase.

BB1_BB2_PNG.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1108
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 772
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 621
    Illegal SapI.rc site found at 831


[edit]
Categories
Parameters
None