Part:BBa_K1520020

Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter

Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter

It produces luxR-AHL complex when there is enough IPTG.

From left to right, Marker(1Kb), Pcons2-rbs-lacI-T-Plac-rbs-luxI-T, Pcons2-rbs-lacI-T-Plac-rbs-luxI-T-Pcons2-rbs-luxR-Ter. The substances of electrophoresis are made from PCR of plasmid with VF2 and VR as their primers.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NheI site found at 2380
  Illegal NheI site found at 2403
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1208
  Illegal BglII site found at 2222
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]