Coding
Part:BBa_K1321337:Design
Designed by: Chris N Micklem Group: iGEM14_Imperial (2014-10-08)
sfGFP in Freiburg format (RFC 25)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 10
Design Notes
OEPCR
Source
Made from the existing part [BBa_I746909]
References
Origin of sfGFP sequence: Pedelacq et al (2006): "Engineering and characterization of a superfolder green fluorescent protein", Nature Biotech 24 (1) January 2006