Part:BBa_K1537016

GSG linker+P2A

P2A

2A peptide sequences were found in Picornaviruses to mediate "cleavage" between two proteins. We use 2A peptide-linked multicistronic vectors to express multiple proteins from a single open reading frame (ORF) effectively. To minimize the risk of homologous recombination, it is important to use different 2A peptide sequences if more than two genes are being linked. The 2A peptide system has thus far worked successfully in all eukaryotic systems tested, from mammalian cells, yeast, and plants.(A El et al，2004)

GSG linker

GSG linker is an oligopeptide of “Gly-Ser-Gly” between your protein and 2A peptide to enhance cleavage.( Andrea L et al，2012)

References

Abdelhak El Amrani,Abdellah Barakate,Barak M. Askari, Xuejun Li, Alison G. Roberts,Martin D. Ryan, and Claire Halpin.(2004)Coordinate Expression and Independent Subcellular Targeting of Multiple Proteins from a Single Transgene.Plant Physiology.Vol. 135, pp. 16–24

Andrea L. Szymczak-Workman, Kate M. Vignali, and Dario A.A. Vignali.(2012)Design and construction of 2A vectors.Cold Spring Harb Protoc :199-204.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]